Many methods to estimate amino acid requirement based on amino acid profile of fish have been proposed. This study evaluates the methodology proposed by Meyer & Fracalossi (2005) and by Tacon (1989) to estimate amino acids requirement of fish. which do exempt knowledge on previous nutritional requirement of reference amino acid. Data on amino acid requirement of pacu. https://chefesquipmenters.shop/product-category/pull-through-knife-sharpeners/